LabTAU seminar- High-Frequency and Very-High-Frequency Quantitative Ultrasound for Biomedical Applications in Cancer, Ophthalmology, and Developmental Biology

Author: Jonathan Mamou
Summary: High-frequency ultrasound (HFU, >20 MHz) and scanning acoustic microscopy (SAM, >200 MHz) offer a means of investigating biological tissue at the microscopic level with spatial resolutions better than 100 μm or 10 μm, respectively. After a brief review of conventional ultrasound imaging, this talk will introduce HFU imaging and present two HFU examples and one SAM example.
Novel HFU imaging methods to form three-dimensional (3D) images of the brain of developing mouse embryos: Many genetically-engineered mice display abnormal development of the central nervous system (CNS) at early embryonic ages and HFU offers great potential for in vivo imaging and characterization. A 34 MHz, five-element annular array was excited using chirp-coded excitation. In utero, in vivo 3D data sets were acquired from more than 100 embryos over five stages, from embryonic days E10.5 to E14.5, and CNS volume renderings were obtained.
The second HFU study focuses on 3D imaging and characterization of lymph nodes freshly-excised from cancer patients. Quantitative-ultrasound (QUS) images were formed and used to detect metastases using a transducer that has a 26-MHz center frequency. Classification results suggest that these QUS methods may provide a clinically-important means of identifying small metastatic foci that might not be detected using standard pathology procedures.
The SAM study focuses on using a novel microscopy system as well as dedicated signal-processing methods to form quantitative images of thin sections of tissue using transducers ranging from 250 MHz to 500 MHz. These techniques yield spatial resolutions better than 8 μm and can be used to assess morphological, acoustical and mechanical tissue properties quantitatively. Data obtained from excised eyes from a guinea pig model of myopia will be presented.
Time:11:00
Place:Salle de réunion duLabTAU u1032 151 Cours A.Thomas , Lyon